Improved PCR-RFLP for the Detection of Diminazene Resistance in Trypanosoma congolense under Field Conditions Using Filter Papers for Sample Storage
نویسندگان
چکیده
1 Centre International de Recherche-Développement sur l’Elevage en zone Subhumide (CIRDES), Bobo Dioulasso, Burkina Faso, 2 Institute for Parasitology and Tropical Veterinary Medicine, Freie Universität Berlin, Berlin, Germany, 3 Central Veterinary Laboratories, Pong-Tamale, Tamale, Ghana, 4 International Livestock Research Institute (ILRI), Bamako, Mali, 5 Laboratoire Central Vétérinaire (LCV), Bamako, Mali, 6 Pan African Tsetse and Typanosomosis Eradication Programme (PATTEC), Bamako, Mali, 7 International Livestock Research Institute (ILRI), Nairobi, Kenya, 8 Institut of Tropical Medicine, Antwerp, Belgium, 9 Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa
منابع مشابه
Five-fold increase in Trypanosoma congolense isolates resistant to diminazene aceturate over a seven-year period in Eastern Zambia.
Two groups of Trypanosoma congolense isolates collected from cattle in 1996 (n=39) and 2003 (n=38) in the Eastern Province of Zambia were analyzed by BclI-PCR-RFLP to assess the evolution of diminazene aceturate (DA) resistance over a period of seven years. The results show a significant increase of DA resistance in this relatively short period of time. In 1996, among the 39 isolates, 61.5% wer...
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A cross-sectional study was carried out in the Ghibe valley from August to October 2010. 411 head of cattle were sampled in eight villages for buffy coat examination (BCE) and blood spots were collected from each animal for trypanosomose diagnosis by 18S-PCR-RFLP and diminazene aceturate (DA) resistance by Ade2-PCR-RFLP. Three villages were selected in a zone where trypanosomosis control operat...
متن کاملHigh Prevalence of Drug Resistance in Animal Trypanosomes without a History of Drug Exposure
BACKGROUND Trypanosomosis caused by Trypanosoma congolense is a major constraint to animal health in sub-Saharan Africa. Unfortunately, the treatment of the disease is impaired by the spread of drug resistance. Resistance to diminazene aceturate (DA) in T. congolense is linked to a mutation modifying the functioning of a P2-type purine-transporter responsible for the uptake of the drug. Our obj...
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From May to November 2005, a study was carried out to assess the occurrence of trypanocidal drug resistance (DR) in trypanosomes of naturally infected cattle of the Adamaoua region of Cameroon. Two distinct Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) procedures were used together with an Allele specific-PCR (AS-PCR) and the standardized single-dose mouse test. ...
متن کاملProtective efficacy of isometamidium chloride and diminazene aceturate against natural Trypanosoma brucei, Trypanosoma congolense and Trypanosoma vivax infections in cattle under a suppressed tsetse population in Uganda.
The protective efficacy of isometamidium chloride (ISMM) and diminazene aceturate (DIM) against Trypanosoma brucei, Trypanosoma congolense and Trypanosoma vivax infections in cattle under a suppressed tsetse population was assessed in southeast Uganda. A total of 66 and 57 trypanosome-infected cattle were treated with ISMM and DIM, respectively together with 177 trypanosome-free animals not tre...
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عنوان ژورنال:
دوره 5 شماره
صفحات -
تاریخ انتشار 2011